Gene Cloning Quiz
Preview:
Restriction enzymes….
A. Cut DNA at a specific site
B. Can be used to isolate a gene of interest
C. Can be used to cut vector DNA
D. Do all of these things
Combining DNA from 2 different organisms is called
A. Bioengineering
B. Biotechnology
C. Recombinant DNA
D. Genetic Engineering
_______ cells are used to produce human proteins at an increased rate for gene therapy.
A. Plant
B. Human
C. Animal
D. Bacteria
In order to insert a human gene into plasmid, both must
A. code for the same gene product.
B. be cut by the same restriction enzyme.
C. originate from the same type of cell.
D. have identical sequences.
What type of macromolecule are restriction enzymes?
A. nucleic acid
B. carbohydrate
C. protein
D. lipid
_________ DNA is created by using the ______ from a bacteria and splicing a section of DNA into it.
A. recombinant, plasmid
B. Cloning, ribosome
C. transcription, translation
D. cumulus, mitochondria
Plasmids are found in bacteria. Scientists use plasmids to
A. Insert eukaryotic genes into bacteria
B. insert eukaryotic genes into humans
C. insert clones into the parent organism
How do scientists cut DNA into smaller strands?
A. restriction enzymes
B. scissors
C. ligase
D. agarose
The first step to genetic engineering is:
A. isolate gene of interest
B. Cut DNA strand
C. Reconnect DNA fragements
D. Produce proteins
Which enzyme “glues” DNA fragments together?
A. restriction enzymes
B. DNA ligase
C. DNA polymerase
D. DNA helicase
Which technology below would probably be the most important to a person who had diabetes and had to take insulin every day?
A. using recombinant DNA to produce human hormones from bacteria
B. testing parents for genetic disorders before they have children
C. engineering fruits and vegetables that resist insects and other pests
D. developing ways to identify criminals through DNA fingerprinting
When DNA is manipulated and moved from one source to another it is known as
A. genetic engineering
B. electrophoresis
C. gene therapy
D. GMO
Which of the following could be used as a vector for a human gene?
A. Bacterial Plasmid
B. Bacteria
C. A mitochondria
D. mRNA
Where is DNA located in a bacterial cell?
A. nucleus
B. ribosomes
C. cell membrane
D. cytoplasm
The process of putting recombinant DNA into an organism is called….
A. Transformation
B. Ligating
C. Electrophoresis
D. Incubating
