Proteomics and Immunology Quiz
Preview:
What is the first step in the ELISA procedure?
A. Incubation of detection antibody
B. Immobilization of the capture antibody
C. Adding stop solution
D. Adding analyte into the well
What is the standard curve equation used for in ELISA?
A. To identify the type of antigen
B. To measure the temperature
C. To calculate the absorbance
D. To determine the unknown concentration of samples
Which of the following is NOT a type of antigen?
A. Exogenous antigen
B. Endogenous antigen
C. Cytokine
D. Autoantigen
What is the absorbance measurement for severe inflammation in IL-6 testing?
A. 10 pg/ml
B. < 1.85 pg/ml C. > 35 pg/ml
D. 20 pg/ml
What is the most abundant circulating antibody in the human body?
A. IgA
B. IgE
C. IgM
D. IgG
What is the role of streptavidin-HRP in the ELISA test?
A. To amplify the signal
B. To immobilize the capture antibody
C. To stop the enzyme reaction
D. To bind to the analyte
What type of antigen is generated inside the body due to infections?
A. Tumor antigen
B. Autoantigen
C. Endogenous antigen
D. Exogenous antigen
What does the ‘Fab’ in antibody structure stand for?
A. Fragment antibody-blocking
B. Fragment antigen-binding
C. Fragment antibody-binding
D. Fragment antigen-blocking
Which of the following is a method for protein detection?
A. Mass Spectrometry
B. PCR
C. Western Blotting
D. Gel Electrophoresis
What is the purpose of the ELISA test?
A. To measure blood pressure
B. To detect antibodies or antigens
C. To analyze DNA sequences
D. To perform a complete blood count
What is the significance of the paratope in antibodies?
A. It is a type of antigen
B. It enhances the immune response
C. It stabilizes the antibody structure
D. It binds to the antigen
What is the role of Interleukin-6?
A. To activate innate immune response
B. To inhibit inflammation
C. To enhance blood clotting
D. To promote apoptosis
What is the molecular mass range of antigens?
A. 100,000 to 1,000,000 Da
B. 1,000 to 10,000 Da
C. 10,000 to 100,000 Da
D. 14,000 to 6,000,000 Da
What is the purpose of the stop solution in an ELISA test?
A. To enhance the color reaction
B. To stop the enzyme-substrate reaction
C. To dilute the sample
D. To immobilize the antibody
What does the ‘Fc’ in antibody structure refer to?
A. Fragment crystallizable
B. Fragment complement
C. Fragment constant
D. Fragment cell-binding
