LB containing ampicillin (2nd plate) is inoculated with the E. coli bacteria that contains ampicillin resistant gene. The bacteria will;
A. Grow on the agar plate
B. Will not grow on the agar plate.
C. will only grow after the plate has been incubated at 45C
D. will only grow after the plate has been placed in a warm water bath for 2 minutes.
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Which of the following describes how a plasmid and gene of interest are combined to create a vector?
A. A restriction enzyme binds to the same restriction site on both DNA strands then DNA ligase seals phosphodiester linkages
B. A primer binds to the gene of interest to facilitate the process of inserting the gene into the plasmid
C. A helicase unwinds the DNA to allow for the gene of interest to be inserted then DNA ligase seals phosphodiester linkages
D. A DNA polymerase uses the gene of interest as a template to insert the gene into the plasmid
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