Biotechnology Quiz
Preview:
An enzyme that cut DNA molecules at a specific “recognition site” is called
A. Restriction enzyme
B. DNA polymerase
C. RNA polymerase
D. Taq polymerase
Based on these results whose blood was found in the blood stain at the crime scene?
A. Bob
B. Sue
C. John
D. Lisa
What happens during the Anneal step of PCR?
A. Primers are created.
B. The primers attach to the target DNA region.
C. Primers copy the new DNA strand.
D. Primers sequence DNA
Why does the DNA move towards positive end?
A. Deoxyribose is negatively charged
B. Deoxyribose is positively charged
C. Phosphate is negatively charged
D. Phosphate is positively charged
What cuts the DNA prior to running gel electrophoresis?
A. DNA polymerase
B. Helicase
C. Ligase
D. Restriction enzyme
At what temperature does the Extension step of PCR occur?
A. 95°C
B. 50-65°C
C. 72°C
D. Any temperature
Which of the following describes why Taq polymerase is required for PCR?
A. The Taq polymerase is able to unwind the DNA double helix reducing the need of additional enzymes
B. The Taq polymerase binds to the DNA without a required primer facilitating the process of PCR
C. The process of PCR requires prokaryotic polymerase as it is less complex
D. The process of PCR requires extremely high temperatures that would denature other polymerases
Which of the following describes why some bands are darker in a gel electrophoresis?
A. This is a location where there is a secondary well in the gel
B. There are large amounts of fragments the same size
C. The DNA at that band absorbed more dye since it is more positively charged
D. This is an error in the gel electrophoresis caused by a clogged pore in the gel
In order to insert a human gene into plasmid, both must
A. code for the same gene product.
B. be cut by the same restriction enzyme.
C. originate from the same type of cell.
D. have identical sequences.
What is transformation?
A. The use of viruses to transform or genetically engineer cells.
B. The measure of how well cells are transformed in a new phenotype.
C. The insertion of a foreign plasmid into a bacterial cell which results in new acquired traits.
D. Genetic engineering or transformation of mammalian cells.
Bacteria cells can be used to produce insulin for the treatment of diabetes in humans. Which best explains how the bacteria cells are able to produce insulin for humans?
A. The bacteria have been grown on a petri dish containing insulin.
B. The bacteria have been genetically engineered to contain the DNA needed to produce insulin.
C. The bacteria have been exposed to radioactivity to create mutant strains capable of producing insulin.
D. The bacteria have been grown on a petri dish containing sugar; the bacteria produce insulin in response to the sugar.
Which use of biotechnology most benefits North Carolina agriculture?
A. producing human vaccines
B. producing disease resistant crops
C. producing new medicines from bacteria
D. producing enzymes to eliminate pollutants
Which of the following describes the orientation of the DNA found in a run gel electrophoresis?
A. Small fragments are found near the well since it is negatively charged
B. Large fragments are found near the well while small fragments are found at the other end
C. Small fragments are found near the well while large fragments are found at the other end
D. Large fragments are found near the well since it is positively charged
Which DNA tech involves adding plasmid to prokaryote?
A. Gel electrophoresis
B. PCR
C. Sequencing
D. Transformation
A technique for making millions of copies of a specific region of DNA.
A. DNA ligase
B. restriction enzymes
C. gel electrophoresis
D. polymerase chain reaction
What happens in the Denature step of PCR?
A. The DNA nucleotides are broken apart.
B. The base-pairing rules for DNA are reversed.
C. The double-stranded DNA is separated into two single strands of DNA.
D. The DNA is returned to its natural setting.
LB containing ampicillin (2nd plate) is inoculated with the E. coli bacteria that contains ampicillin resistant gene. The bacteria will;
A. Grow on the agar plate
B. Will not grow on the agar plate.
C. will only grow after the plate has been incubated at 45C
D. will only grow after the plate has been placed in a warm water bath for 2 minutes.
Which is the primary purpose of using restriction enzymes in gel electrophoresis?
A. It allows the strands of DNA to be cut into various lengths for testing
B. It restricts the number of base pairs that can be tested in a sample
C. It makes the testing simpler by moving the strands into the gel faster
D. It charges the DNA strands
This is the practice of inserting the DNA of another species into an organism’s DNA so that the organism gains a new trait.
A. Cloning
B. Gel Electrophoresis
C. Genetic Engineering
D. DNA Fingerprinting
What does PCR stand for?
A. Place for cellular respiration
B. Primase cytosine reactants
C. Polymerase chain reaction
D. Preferred chemical reaction
At what temperature does the Anneal step of PCR occur?
A. 95° C
B. 50° C
C. 72° C
D. Any temperature
Heat shock
A. Trap the plasmids inside the cell.
B. Enlarges the pores of the cell.
C. Provides for the death of the cell.
D. Increases the disintegration of the cell wall.
Which suspect was at crime scene?
A. A
B. B
C. C
D. D
Which is the primary purpose of using restriction enzymes in gel electrophoresis?
A. It allows the strands of DNA to be cut into various lengths for testing
B. It restricts the number of base pairs that can be tested in a sample
C. It makes the testing simpler by moving the strands into the gel faster
D. It charges the DNA strands
If one fragment was placed in the thermocycler for a PCR experiment, how many fragments would exist after 5 cycles?
A. 10
B. 16
C. 64
D. 32
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