Biotechnology Quiz
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Which is the primary purpose of using restriction enzymes in gel electrophoresis?
A. It allows the strands of DNA to be cut into various lengths for testing
B. It restricts the number of base pairs that can be tested in a sample
C. It makes the testing simpler by moving the strands into the gel faster
D. It charges the DNA strands
What happens in the Denature step of PCR?
A. The DNA nucleotides are broken apart.
B. The base-pairing rules for DNA are reversed.
C. The double-stranded DNA is separated into two single strands of DNA.
D. The DNA is returned to its natural setting.
LB containing ampicillin (2nd plate) is inoculated with the E. coli bacteria that contains ampicillin resistant gene. The bacteria will;
A. Grow on the agar plate
B. Will not grow on the agar plate.
C. will only grow after the plate has been incubated at 45C
D. will only grow after the plate has been placed in a warm water bath for 2 minutes.
Which DNA tech separates fragments by size/charge?
A. Gel electrophoresis
B. PCR
C. Sequencing
D. Transformation
Which use of biotechnology most benefits North Carolina agriculture?
A. producing human vaccines
B. producing disease resistant crops
C. producing new medicines from bacteria
D. producing enzymes to eliminate pollutants
In order to insert a human gene into plasmid, both must
A. code for the same gene product.
B. be cut by the same restriction enzyme.
C. originate from the same type of cell.
D. have identical sequences.
This is the practice of inserting the DNA of another species into an organism’s DNA so that the organism gains a new trait.
A. Cloning
B. Gel Electrophoresis
C. Genetic Engineering
D. DNA Fingerprinting
What is the function of a primer?
A. To identify the particular region of DNA to be copied by PCR.
B. To copy DNA.
C. To create DNA nucleotides.
D. To maintain the temperature of the PCR reaction.
What is transformation?
A. The use of viruses to transform or genetically engineer cells.
B. The measure of how well cells are transformed in a new phenotype.
C. The insertion of a foreign plasmid into a bacterial cell which results in new acquired traits.
D. Genetic engineering or transformation of mammalian cells.
The unpaired nucleotides produced by the action of restriction enzymes are referred to as…..
A. sticky end.
B. base sequence.
C. single strands.
D. restriction fragments.
Observe the results above. What gene is present in the engineered plasmid?
A. kanamycin resistant gene
B. ampicillin resistant gene
C. tetracycline resistant gene
D. No plasmid is necessary to grow on plate 3.
Why does PCR require a specific type of polymerase?
A. Taq polymerase can withstand the range of temperatures in PCR without denaturing.
B. Taq polymerase is the most inexpensive type of polymerase.
C. Taq polymerase is the most common type of polymerase.
D. A specific type of polymerase is unnecessary.
The process of changing the genetic material of a living organism
A. Genetic Modification
B. Taxonomy
C. Adapting
D. Bioremediation
What does PCR stand for?
A. Place for cellular respiration
B. Primase cytosine reactants
C. Polymerase chain reaction
D. Preferred chemical reaction
Bacteria cells can be used to produce insulin for the treatment of diabetes in humans. Which best explains how the bacteria cells are able to produce insulin for humans?
A. The bacteria have been grown on a petri dish containing insulin.
B. The bacteria have been genetically engineered to contain the DNA needed to produce insulin.
C. The bacteria have been exposed to radioactivity to create mutant strains capable of producing insulin.
D. The bacteria have been grown on a petri dish containing sugar; the bacteria produce insulin in response to the sugar.
At what temperature does the Denature step of PCR occur?
A. 95° C
B. 50° C
C. 72° C
D. Any temperature
Which two bear species are most closely related?
A. Bear 1 and Bear 2
B. Bear 1 and Bear 3
C. Bear 2 and Bear 3
D. There’s no way to tell
Which DNA tech involves adding plasmid to prokaryote?
A. Gel electrophoresis
B. PCR
C. Sequencing
D. Transformation
Which of the following describes how a plasmid and gene of interest are combined to create a vector?
A. A restriction enzyme binds to the same restriction site on both DNA strands then DNA ligase seals phosphodiester linkages
B. A primer binds to the gene of interest to facilitate the process of inserting the gene into the plasmid
C. A helicase unwinds the DNA to allow for the gene of interest to be inserted then DNA ligase seals phosphodiester linkages
D. A DNA polymerase uses the gene of interest as a template to insert the gene into the plasmid
Which is the primary purpose of using restriction enzymes in gel electrophoresis?
A. It allows the strands of DNA to be cut into various lengths for testing
B. It restricts the number of base pairs that can be tested in a sample
C. It makes the testing simpler by moving the strands into the gel faster
D. It charges the DNA strands
At what temperature does the Anneal step of PCR occur?
A. 95° C
B. 50° C
C. 72° C
D. Any temperature
DNA fingerprinting works because
A. Most genes are dominant
B. All organisms contain RNA
C. The most important genes are different among most people
D. No two people, except identical twins, have exactly the same DNA
Why does the DNA move towards positive end?
A. Deoxyribose is negatively charged
B. Deoxyribose is positively charged
C. Phosphate is negatively charged
D. Phosphate is positively charged
A technique for making millions of copies of a specific region of DNA.
A. DNA ligase
B. restriction enzymes
C. gel electrophoresis
D. polymerase chain reaction
Which of the following describes why some bands are darker in a gel electrophoresis?
A. This is a location where there is a secondary well in the gel
B. There are large amounts of fragments the same size
C. The DNA at that band absorbed more dye since it is more positively charged
D. This is an error in the gel electrophoresis caused by a clogged pore in the gel
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