Biotechnology Quiz
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What has the Rough ER attached to it?
A. Nucleolus
B. Golgi apparatus
C. Ribosomes
D. Chromosomes
Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?
A. DNA is negatively charged and attracted to the positive end of the unit
B. DNA is positively charged and attracted to the negative end of the unit
C. the agarose gel is negatively charged
D. the agarose gel is positively charged
What is the small dark structure in the nucleus that produces ribosomes?
A. Rough ER
B. Nucleolus
C. Smooth ER
D. Chromatin
Which organelle are sac-like tunnels that have ribosomes attached and whose main function is to make and process proteins?
A. smooth endoplasmic reticulum
B. rough endoplasmic reticulum
C. golgi apparatus
D. vesicles
Which DNA tech involves adding plasmid to prokaryote?
A. Gel electrophoresis
B. PCR
C. Sequencing
D. Transformation
Based on these results whose blood was found in the blood stain at the crime scene?
A. Bob
B. Sue
C. John
D. Lisa
At what temperature does the Denature step of PCR occur?
A. 95° C
B. 50° C
C. 72° C
D. Any temperature
How many cycles of denaturation, annealing, and extension are typically performed in a PCR reaction?
A. 1 cycle
B. 5 cycles
C. 20-40 cycles
D. 90-100 cycles
Which use of biotechnology most benefits North Carolina agriculture?
A. producing human vaccines
B. producing disease resistant crops
C. producing new medicines from bacteria
D. producing enzymes to eliminate pollutants
Which of the following is the “powerhouse” of the cell that breaks down food into energy that the cell needs?
A. Mitochondria
B. Vacuole
C. Endoplasmic reticulum
D. Chloroplast
Which of the following is a saclike structure that stores water?
A. Golgi apparatus
B. Nucleus
C. Vacuole
D. Endoplasmic reticulum
Which of the following organelles is long hollow, beaded tubular structure that facilitates the movement of other cell organelles within the cell through binding proteins?
A. microtubule
B. microvilli
C. microfilament
D. mitochondria
Which two bear species are most closely related?
A. Bear 1 and Bear 2
B. Bear 1 and Bear 3
C. Bear 2 and Bear 3
D. There’s no way to tell
What is the purpose of gel electrophoresis?
A. To cut DNA at specific recognition sites
B. To join fragments of DNA
C. To amplify DNA sequences
D. To separate DNA fragments according to size
What happens in the Denature step of PCR?
A. The DNA nucleotides are broken apart.
B. The base-pairing rules for DNA are reversed.
C. The double-stranded DNA is separated into two single strands of DNA.
D. The DNA is returned to its natural setting.
Which of the following describes why some bands are darker in a gel electrophoresis?
A. This is a location where there is a secondary well in the gel
B. There are large amounts of fragments the same size
C. The DNA at that band absorbed more dye since it is more positively charged
D. This is an error in the gel electrophoresis caused by a clogged pore in the gel
An enzyme that cut DNA molecules at a specific “recognition site” is called
A. Restriction enzyme
B. DNA polymerase
C. RNA polymerase
D. Taq polymerase
Which of the following describes how a plasmid and gene of interest are combined to create a vector?
A. A restriction enzyme binds to the same restriction site on both DNA strands then DNA ligase seals phosphodiester linkages
B. A primer binds to the gene of interest to facilitate the process of inserting the gene into the plasmid
C. A helicase unwinds the DNA to allow for the gene of interest to be inserted then DNA ligase seals phosphodiester linkages
D. A DNA polymerase uses the gene of interest as a template to insert the gene into the plasmid
Which is the primary purpose of using restriction enzymes in gel electrophoresis?
A. It allows the strands of DNA to be cut into various lengths for testing
B. It restricts the number of base pairs that can be tested in a sample
C. It makes the testing simpler by moving the strands into the gel faster
D. It charges the DNA strands
Which of the following is NOT contained in a chloroplast?
A. DNA
B. chlorophyll
C. ribosomes
D. nucleus
Which organelle is the “packing center” for the cell?
A. Endoplasmic reticulum
B. Nucleus
C. Lysosome
D. Golgi apparatus
Which of the following is NOT found in prokaryotes?
A. DNA or the genetic material
B. A cell membrane
C. A nucleus
D. Cytoplasm
Eukaryotic cells contain____.
A. only ribosomes
B. Membrane bound organelles
C. DNA floating in cytoplasm
D. only cytoplasm
Which of the following describes why Taq polymerase is required for PCR?
A. The Taq polymerase is able to unwind the DNA double helix reducing the need of additional enzymes
B. The Taq polymerase binds to the DNA without a required primer facilitating the process of PCR
C. The process of PCR requires prokaryotic polymerase as it is less complex
D. The process of PCR requires extremely high temperatures that would denature other polymerases
What is the goal of PCR?
A. To cut DNA at specific recognition sites
B. To join fragments of DNA
C. To amplify a specific DNA region
D. To separate DNA fragments according to size
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