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Biotechnology › View

Which of the following describes how a plasmid and gene of interest are combined to create a vector?

A. A restriction enzyme binds to the same restriction site on both DNA strands then DNA ligase seals phosphodiester linkages

B. A primer binds to the gene of interest to facilitate the process of inserting the gene into the plasmid

C. A helicase unwinds the DNA to allow for the gene of interest to be inserted then DNA ligase seals phosphodiester linkages

D. A DNA polymerase uses the gene of interest as a template to insert the gene into the plasmid

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Biotechnology › View

What happens during the Anneal step of PCR?

A. Primers are created.

B. The primers attach to the target DNA region.

C. Primers copy the new DNA strand.

D. Primers sequence DNA

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