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Which is the correct order of making phylogenetic tree?

A. sequencing –> alignment –> amplification –> tree construction

B. tree construction –> sequencing –> alignment –> amplification

C. amplification –> sequencing–> alignment –> tree construction

D. amplification –> alignment –> sequencing –> tree construction

The erythrocytosis seen in relative polycythemia occurs because of:

A. decreased arterial oxygen saturation

B. decreased plasma volume of circulating blood

C. increased erythropoietin levels

D. increased erythropoiesis in the bone marrow