Plasmid Quiz

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plasmid pBR322 is developed by

A. Beadle and Tatum

B. Watson and Crick

C. Bolivar and Rodriguez

D. Jacob and Monad

In cloning vector, the antibiotics resistant gene are used

A. As sequences from where replication starts

B. To keep the cultures free of infection

C. As selectable markers

D. To select healthy vectors

Selectable marker are used to?

A. Differentiate b/w trasnformant and non transformant

B. highlight writeen text

A cloning vector must have

A. Promoter

B. Selectable marker

What are selectable marker ?

A. a radium labelled marker

B. thick pen

C. resistant gene

D. a type of protein

The term molecular scissors generally refers to

A. DNA polymerases

B. RNA polymerases

C. Restriction endonucleases

D. DNA ligases

What are the features of good cloning vector?

A. high number of recognition site

B. very few number of recognition site

The foreign DNA is cloned at

A. Anywhere in the vector

B. At a unique restriction site

C. Both 1 and 2

D. None of the above

A cloning vector must possess?

A. ori

B. selectable marker

C. both of above

D. non of these

what is origin of replication ?

A. place of origin

B. sequence of genome where replication stars

C. where DNA are formed

D. Where RNA are formed

what is plasmid?

A. genetic material

B. selfreplicating extrachromosomal genetic material

C. double stranded DNA

D. Genetic material of a Virus

Name the antibiotics resistant gene present in pBR322.

A. kanamycin

B. streptomycin

C. tetracycline

D. chloramphenicol

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