Which of the following describes how a plasmid and gene of interest are combined to create a vector?

A. A restriction enzyme binds to the same restriction site on both DNA strands then DNA ligase seals phosphodiester linkages

B. A primer binds to the gene of interest to facilitate the process of inserting the gene into the plasmid

C. A helicase unwinds the DNA to allow for the gene of interest to be inserted then DNA ligase seals phosphodiester linkages

D. A DNA polymerase uses the gene of interest as a template to insert the gene into the plasmid